Effects of co-substrate proportions on methane production and microbial community involved in fermentable household waste and manure co-digestion - A molecular ecology approach using Stable Isotope Probing and metagenomics to study viruses of methanogens



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15/11/19 - Séminaires de Franciele PEREIRA CAMARGO (University of São Paulo, Brazil) et Ariane BIZE (Irstea Antony)

Le vendredi 15 novembre 2019 à 13h00, salle B. Auvray, bâtiment 15, Campus de Beaulieu, UR1

Effects of co-substrate proportions on methane production and microbial community involved in fermentable household waste and manure co-digestion

Anaerobic digestion is an interesting option to reduce the environmental impact of organic waste treatment name by reducing waste mass while producing renewable energy, i.e. methane-rich biogas. A current issue is thus to optimize the co-digestion of various types of waste in order to prevent inhibition problems and to achieve high process performance. The present study aimed at evaluating different operational parameters for the co-digestion of fermentable household waste, manure and wastewater treatment sludge, focusing on how the proportions of co-substrates affect methane production and microbial community composition. In this purpose, 16S rRNA gene metabarcoding data of samples from dry batch anaerobic reactors (60 liters) were analyzed.

Contact : Franciele PEREIRA CAMARGO

A molecular ecology approach using Stable Isotope Probing and metagenomics to study viruses of methanogens namediversity

Viruses of microbes are ubiquitous and thus represent major drivers of ecosystem functioning. Viral diversity of anaerobic digestion plants remains still poorly documented. To our knowledge, a unique publication has been dedicated to this metavirome sequencing. Our study focuses on the in situ diversity of DNA viruses of methanogenic archaea. We are developing an original approach based on stable isotope probing (SIP) and high throughput sequencing. SIP consists in separating DNA molecules according to their density by ultracentrifugation on a CsCl gradient. Using various 13C-labeled methanogenesis substrates we selectively activated distinct populations of methanogens and enriched them in 13C. The viruses that infected these microbes were also enriched and could be characterized.

Contact : Ariane BIZE





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